Table 1.
5′→3′ | Nucleotide position | |
Primers for cDNA synthesis | ||
CHIKV | ACGAAACCACTGTATCACAGCG | 921-900 |
MAYV | CGGTTTCATTCTCTTCTTCCTC | 781-760 |
Primers for qPCR | ||
CHIKV F | CGTACTGTTCTCAGTCGGGTC | 791-811 |
CHIKV R | GATGGAACACTGAAGGTAAGTGC | 873-851 |
MAYV F | CCTACCCAACATATGCAACCA | 648-668 |
MAYV R | AAGGTGTCCCTCAGTCAGT | 742-724 |
Probes | ||
CHIKV | TACCCGGAGAGCCGTAAGCTTCTTA | 819-843 |
MAYV | ACGAACAGGTGTTGAAAGCCAGGA | 678-701 |