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. 2019 Jan 22;56(3):832–843. doi: 10.1093/jme/tjy241

Table 1.

Oligonucleotide primers and DNA probes for the specific detection of MAYV and CHIKV in mixed infections

5′→3′ Nucleotide position
Primers for cDNA synthesis
CHIKV ACGAAACCACTGTATCACAGCG 921-900
MAYV CGGTTTCATTCTCTTCTTCCTC 781-760
Primers for qPCR
CHIKV F CGTACTGTTCTCAGTCGGGTC 791-811
CHIKV R GATGGAACACTGAAGGTAAGTGC 873-851
MAYV F CCTACCCAACATATGCAACCA 648-668
MAYV R AAGGTGTCCCTCAGTCAGT 742-724
Probes
CHIKV TACCCGGAGAGCCGTAAGCTTCTTA 819-843
MAYV ACGAACAGGTGTTGAAAGCCAGGA 678-701