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(A) We incubated M23-AQP4–overexpressing CHO cells with 10% serumNMOSD and 2.5% hC with or without 500 μg/mL of C5B3 for 30 minutes and fixed and stained them with anti–C5b-9 antibody. (B) We considered the red-to-blue fluorescence ratio to be statistically significant. Scale bar is 200 μm. We used a 2-tailed unpaired t test to compare the 2 groups; ***p < 0.001. AQP4 = aquaporin-4; hC = human complement; MAC = membrane attack complex.
