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. Author manuscript; available in PMC: 2019 Dec 14.
Published in final edited form as: ACS Infect Dis. 2018 Oct 22;4(12):1737–1745. doi: 10.1021/acsinfecdis.8b00222

Figure 3.

Figure 3.

Inhibition of MukB in vitro. (A) Inhibition of MukB-mediated DNA bridging. MukB is bound to a bead-tethered DNA, and another DNA is then added to be captured and recovered by MukB in the presence of the indicated amounts of the hit compounds. (B) The amount of MukB-captured DNA in the presence of the indicated compounds (n ≥ 2, ±SD). (C) Michellamine-induced protein aggregation. Following 10 min of incubation, the aggregated protein was precipitated by low speed centrifugation, and the top and bottom fractions resolved by gel electrophoresis. (D) Percent of unaggregated MukB found in the top fraction after incubation with the indicated compounds (n ≥ 2, ±SD).