Identification of fatty acid desaturation products in transgenic
yeast. FAMEs were extracted from yeast transformed with pYESZFB10 grown
in the presence of 18:2n-6 (A),
18:3n-3 (B), 20:3n-6
(C), or 20:4n-3 (D). The
first four peaks in A–D are 16:0,
16:1n-7, 18:0, and 18:1n-9, respectively.
The fifth peaks in each panel are the exogenously added fatty acids
18:2n-6 (A), 18:3n-3
(B), 20:3n-6 (C), and
20:4n-3 (D), respectively. The sixth
peaks in each panel (arrowed) were identified tentatively (based on
retention times) as 18:3n-6 (A),
18:4n-3 (B), 20:4n-6
(C), and 20:5n-3 (D),
respectively.