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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Psychiatr Res. 2019 Jan 10;111:8–15. doi: 10.1016/j.jpsychires.2019.01.007

Fig. 3. Interaction between myospryn and IF proteins, desmin, vimentin, and peripherin.

Fig. 3.

a) Mouse and human desmin, peripherin, and vimentin alignment using COBALT Constraint-based Multiple Protein Alignment Tool. The minimum binding region of desmin (GenBank™ accession number P31001) to myospryn is aa 58–103 and located within the filament head domain (aa 9–105). The red color in the protein alignment indicates highly conserved residues and blue indicates less conserved residues determined using 2 Bits Conservation Setting. b) Summary of Y2H Colony-Lift Filter Assay, where the blue indicates positive interaction, and white indicates no interaction. Desmin interacts with both alleles of myospryn and with both short (aa 4039–4069) and long (aa 3859–4069) myospryn clones, whereas vimentin and peripherin did not show any interaction.