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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Cancer Res. 2019 Mar 12;79(8):1967–1980. doi: 10.1158/0008-5472.CAN-18-1787

Figure 1. MYC overexpression alone drives tumorigenesis from Sox2+ cells in the postnatal cerebellum.

Figure 1.

(A–C) Tumors generated from total cerebellar cells. (A) Bioluminescent imaging. (B) Survival curve. (C) H&E staining of tumor sections. Asterisks show an area of necrosis and a large tumor cell with marked nuclear atypia (anaplasia). Arrow shows prominent nuclear molding. Arrowhead shows mitotic figures. Normal indicates normal tissue. Scale bars = 25 µm. (D) FACS-sorting of Sox2+ cells. (E) Survival curve of tumors generated from Sox2+ or Sox2− cells. (F) Schema for identifying tumor cells-of-origin. (G) tdT expression in tumors generated from Sox2-CreERT2/Rosa-CAG-LSL-tdTomato cells treated with or without 4-OHT. Nuclei were counter-stained with DAPI (blue). Scale bars = 1 mm. (H) Flow cytometry analysis of tdT expression in tumors from (G) and summary of percentage of tdT+ tumors.