(A) Isolated and expanded WT ILC2s were cultured in IL-2 + IL-7 + IL-33 for 72hrs with indicated inhibitors and IL1R2 levels were measured by flow cytometry. (B) Isolated and expanded ILC2s were incubated with indicated inhibitors for 30 minutes followed by PMA stimulation for 20 minutes. Statistical quantification of (C) change in % of cells IL1R2+ and (D) change in MFI following PMA stimulation. n.s. P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. one-way ANOVA (A, C, D). Data are representative of two (mean) independent experiments.