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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Blood Rev. 2019 Jan 28;35:7–17. doi: 10.1016/j.blre.2019.01.003

Figure 5: Flow cytometric comparison of human IgG and IgM antibody binding to pig and human RBCs.

Figure 5:

Human sera (n=83) were incubated with RBCs isolated from WT pigs (W), or from pigs lacking Gal (i.e., GTKO) and Neu5Gc (i.e., CMAH-KO) (double-knockout, D) or lacking Gal, Neu5Gc, and Sda (i.e., β4GalNT2-KO) (triple-knockout, T). These sera were also mixed with human (allogeneic) RBCs (H) expressing blood group O. Panels A and B show a summary of IgG and IgM binding to various RBCs, respectively. The data represent median fluorescent intensity (MFI). Panel C summarizes the number of samples from panels A and B where the indicated pRBC MFI is less than the MFI for the human blood group O RBCs. (Reproduced with permission from reference [67])