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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Blood Rev. 2019 Jan 28;35:7–17. doi: 10.1016/j.blre.2019.01.003

Figure 7: Potential novel techniques for transgenic expression in pRBCs lacking nuclei.

Figure 7:

(A) CRISPR/Cas9 could be used to cleave genomic regions of GGTA1 (i.e., Gal) at sites flanking exons that contain the open reading frame (ORF). A replacement construct containing the ORF of CD47 flanked by GGTA1 sequences could be simultaneously introduced to facilitate the replacement of GGTA1 protein coding sequences with CD47. (B) The identical approach to that used in panel A could be repeated to replace codons encoding the CMAH gene with the CD55 ORF.