Table 5:

Values for individual ratios of bulk-associated partition functions in Eq. 20, for cholesterol in a binary CHOL:POPC membrane.

Process	Ratio	Method	Result
apply bulk restraints	$\frac{Z_b^{=}}{Z_b^{\square }}$	Eq. 21 with α = 0.3; nL = 230xCHOL	102xCHOL
decouple from bulk	$\frac{Z_b^{-}{Z}_{\text{g}}^{=}}{Z_b^{=}}$	Fit of AFEP data for $\frac{1}{P_x}$ to Eq. 26; see Figure 3A.	${10}^{-14}{e}^{2.7{\left(1-x_{\text{CHOL}}\right)}^2}$
switch to isotropic restraint	$\frac{Z_{\text{g}}^{○}}{Z_{\text{g}}^{=}}$	Eq. 33 with θR=0.14π,rR= 5Å,zR = 11Å,A= = 3600Å2	10−1
bulk to gas phase	$\frac{Z_b^{-}{Z}_{\text{g}}^{○}}{Z_b^{\square }}$	Product of previous 3 rows	${10}^{-13}{x}_{\text{CHOL}}{e}^{2.7{\left(1-x_{\text{CHOL}}\right)}^2}$