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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Cancer Cell. 2019 Mar 28;35(4):603–617.e8. doi: 10.1016/j.ccell.2019.03.001

Figure 3.

Figure 3.

The CHD1 interactome is enriched for AR cofactors. (A) RIME was performed for H3K4me3 and CHD1 in androgen proficient conditions. Signal to noise ratios of significantly enriched peptides from H3K4me3 were subtracted from those of CHD1 and plotted by biological replicate. Orange: peptides unique to H3K4me3; Blue: unique to CHD1; Purple: common. Green: overlap with known AR interactors (Gottlieb et al., 2012; Mooslehner et al., 2012). (B) STRING analysis of AR (Stelloo et al., 2018a) and CHD1 interactomes in LNCaP cells. (C) Proximity Ligation Assay (PLA) for AR and CHD1 in LNCaP cells (left) and quantification of PLA signal from a single plane, plotted as the number of detected foci per cell (right). One-way Anova, Tukey’s multiple comparisons test: ** p < 0.01, **** p < 0.00001. Data represent +/− SEM. Scalebar 20 μm. See also Table S1–S2.