Tumor cells can be isolated by MACS mouse cell depletion and tdTomato+ primary tumor (PT), scaffold (SCAF), and lung (LUNG) derived tumor cells are evident initially at day 0(A), PT cells begin to attach at day 1 but SCAF and LUNG cells remain in suspension(B), and by day 12 SCAF cells attached and grew out to form ~15 colonies of cells, LUNG cells grew out from ~500 cells and PT from ~105 cells(C). Scale bars represent 25, 50, 100 or 400 um as labeled.