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. Author manuscript; available in PMC: 2020 Feb 14.
Published in final edited form as: J Med Chem. 2018 Dec 5;62(3):1125–1137. doi: 10.1021/acs.jmedchem.8b00513

Figure 5. Effects of 1 are diminished in SHP2 knockout cells while cells harboring hyperactivated SHP2 are more sensitive to 1.

Figure 5.

Figure 5.

(A) Ptpn11 conditional knockout (Ptpn11fl/fl/Ade-Cre+) mouse embryonic fibroblasts (MEFs) and WT (Ptpn11+/+/Ade-Cre+) control cells were infected with adeno-Cre viruses and then treated with 1 at 6 μM or DMSO. Cell growth rates were determined at 24, 48 and 72 hours using a One Solution Cell Proliferation Assay kit. SHP2 levels in knockout and control cells were analyzed by immunoblotting analyses with anti-SHP2 antibody. Blots were stripped and reprobed with anti-β-actin antibody to monitor protein loading. Experiments were performed three times. Similar results were obtained in each. Data shown are the mean ± SD of triplicates from one representative experiment. (B) Ptpn11E76K/+ and Ptpn11+/+ control MEFs were treated with 1 (6 μM). Cell numbers were determined using a One Solution Cell Proliferation Assay kit. Experiments were performed three times. Similar results were obtained in each. Data shown are the mean ± SD of triplicates from one representative experiment.