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. Author manuscript; available in PMC: 2020 Feb 14.
Published in final edited form as: J Med Chem. 2018 Dec 5;62(3):1125–1137. doi: 10.1021/acs.jmedchem.8b00513

Figure 6. Lung cancer cells harboring hyperactivated SHP2 are more sensitive to 1 than lung cancer cells expressing WT SHP2.

Figure 6.

Figure 6.

(A) Human lung cancer cell line H661 carrying the PTPN11N58S/+ mutation and H596 with WT PTPN11 were treated with 1 at the indicated concentrations for 48 hours. DMSO-treated cells were included as negative controls. Cell numbers were determined using a One Solution Cell Proliferation Assay kit. Experiments were repeated three times, similar results were obtained in each experiment. Data are presented as the mean ± SD of triplicates from one representative experiment. (B) H661 cells were treated with 1 at 0, 10, and 20 μM. Apoptotic cells were quantified 24 hours later by FACS using an Annexin V staining kit. Cell cycle changes were determined by propidium iodide staining followed by FACS analyses. Experiments were performed three times. Apoptosis data presented are the mean ± SD of three experiments. Cell cycle profiles are representatives of three independent experiments.