Figure 7. Mouse and JMML patient leukemic cells are more sensitive to 1 than normal control cells.

Bone marrow cells harvested from Ptpn11^E76K/+/Mx1-Cre⁺ and Ptpn11^+/+/Mx1-Cre⁺ mice were plated in methylcellulose medium containing GM-CSF (1.0 ng/mL) and 1 at the indicated concentrations or DMSO. Hematopoietic colonies that were generated were enumerated 7 days later and normalized against the number of colonies derived from WT control cells treated with DMSO. Data are presented as the mean ± SD of three independent experiments. (B) Splenocytes/marrow cells/blood cells from JMML patients with the PTPN11^E76K/+ mutation and apheresis samples from healthy donors were plated in methylcellulose medium containing GM-CSF (1.0 ng/mL) and 1 at the indicated concentrations or control DMSO. Leukemic colonies that were produced were enumerated 14 days later and normalized against the number of colonies derived from the cells treated with DMSO. Three patient samples and three apheresis samples were tested in three independent experiments. Similar results were obtained in each. Data are presented as the mean ± SD of triplicates from one patient sample.