Skip to main content
. Author manuscript; available in PMC: 2020 Jan 16.
Published in final edited form as: ACS Chem Neurosci. 2018 Nov 5;10(1):690–703. doi: 10.1021/acschemneuro.8b00498

Figure 5. Protective role of SUVs on DOPAL-induced oligomerization of Ac-aSyn mutants.

Figure 5.

Ac-aSyn mutants (A53T, A30P, E46K, G51D and H50Q) (50 μM in 20 mM sodium phosphate, pH 7.5, 100 mM NaCl), in the presence of varying concentrations of SUV (0, 2, 4, 6, 8, 10, or 12 mM), were incubated with 500 μM DOPAL for 24 hours at 25 °C, and then the samples analyzed by 15% SDS-PAGE (A-E). On the right, population of monomer and oligomers from Ac-aSyn (WT or mutant) upon the incubation with DOPAL in the absence or the presence of 6 mM SUV. Results represents mean ± standard deviation of three to five independent experiments.