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. 2019 Apr 16;9:6118. doi: 10.1038/s41598-019-42630-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ectopic expression of tkv^CA in CySCs leads to GSC loss. (a) c587^ts control testis. GSCs (white arrowheads) and CySCs (yellow arrowhead) are indicated. (b) c587^ts > tkv^CA testis. The hub is surrounded by CySCs (yellow arrowheads), and no germline cells can be observed (white arrowhead). (c) Quantification of the number of GSCs per testis in control and c587^ts > tkv^CA testes. n = 10–15 testes. (d) Quantification of the number of CySCs with their cell body attaching to the hub in control and c587^ts > tkv^CA testes. n = 10–15 testes. (e) CySC MARCM clones in FRT control. GSCs (white arrowheads) and GFP-marked CySCs (yellow arrowhead) are indicated. (f) tkv^CA-expressing CySC MARCM clones. Some GFP-marked tkv^CA CySCs (yellow arrowheads) tightly attach to the hub, and the number of GSCs per testis is greatly reduced (white arrowhead). (g) Quantification of the number of GSCs per testis in testes carrying FRT control and tkv^CA MARCM clones. n = 10 testes. (h) Quantification of the number of CySCs with their cell body attaching to the hub in testes carrying FRT control and tkv^CA MARCM clones. n = 10 testes. mean ± SEM is shown. **p < 0.01. GFP in green, Vasa in red, Fas3 in yellow, blue indicates DAPI staining for DNA. Scale bars: 10 μm.