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. 2019 Apr 16;9:6161. doi: 10.1038/s41598-019-42450-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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p27 and STMN1 interaction regulates microtubule stabilization and migration of osteosarcoma cells. (a) 143B untransfected controls and cells expressing recombinant wild-type p27 (Myc-DDK-p27), T198A mutant p27 (T198A) and p27 depletion were subjected to immunofluorescence staining with antibodies against β-tubulin and p27. β-tubulin is colored red; p27 is colored green; nuclei were stained with Hoechst dye and are colored blue. Magnification is 63x; scale bars = 25 μm. (b) 143B cells were imaged using differential interference contrast (DIC) microscopy and analyzed for nuclear structural defects. Left panel: Representative image of a polynucleated cell expressing T198A mutant p27. Right plot: Each column represents percentage of nuclear structural defects for 100 cells for indicated experimental groups. *p < 0.5. (c) DIC image of 143B control cell and apoptotic cell expressing T198A mutant p27. Magnification is 63x; scale bars = 25 μm. (d) The matrigel invasion assay was conducted using HOS (left plot) and 143B cells (middle and right plots) expressing recombinant wild-type p27, T198A mutant p27 and reduced p27 (siRNACDKN1B). Data is shown as mean + SE from three experiments; *represents p < 0.05.