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. 2019 Apr 16;9:6114. doi: 10.1038/s41598-019-42273-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of plasma membrane permeabilization and potential in L. (L.) infantum promastigotes treated with compound 2 (190 μM). (A) The entrance of SYTOX Green dye was monitored spectrofluorimetrically (excitation 485 nm and emission 520 nm) every 20 min. Untreated promastigotes and treated with TX-100 (0.5%) were used to achieve minimal and maximal permeabilization, respectively. Fluorescence is reported as percentage relative to time 0 min (0%) and 120 min (100%). At 120 min, the addition of 0.5% TX-100 in all samples is represented. (B) DiSBAC2(3) dye fluorescence was measured by flow cytometry (excitation 488 nm and emission 574 nm) after 1 and 2 h of incubation. Untreated promastigotes and treated with raloxifene (60 μM) were used to achieve minimal and maximal depolarization, respectively. Fluorescence is reported as percentage relative to promastigotes treated with raloxifene (100%). A representative experiment is shown. *p < 0.05, **p < 0.01 e ***p < 0.0001.