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. 2019 Apr 16;9:6114. doi: 10.1038/s41598-019-42273-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of mitochondrial membrane potential in L. (L.) infantum promastigotes treated with compound 2 (190 μM). (A) JC-1 dye fluorescence was measured by flow cytometry (excitation 488 nm and emission 530/574 nm) after 1 and 2 h of incubation. Untreated promastigotes and treated with CCCP (100 μM) were used to achieve minimal and maximal depolarization, respectively. Fluorescence is reported as the ratio between the emission channels BL2/BL1. (B) Evaluation of ATP concentration. ATP was measure spectrofluorimetrically after 1 and 2 h of incubation. Untreated promastigotes and treated with CCCP (25 μM) were employ to achieve minimal and maximal depolarization, respectively. Results are expressed in nM. A representative experiment is shown. ***p < 0.0001.