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. 2019 Apr 16;9:6114. doi: 10.1038/s41598-019-42273-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of cell cycle progression in L. (L.) infantum promastigotes treated with compound 2 (190 μM) by flow cytometry (488 nm excitation and 574 nm emission). Propidium iodide fluorescence was arranged in histograms. Untreated promastigotes and treated with miltefosine (25 μM) were employing to achieve minimal and maximal depolarization, respectively. A representative experiment is shown. (A) Untreated control, (B) miltefosine, and (C) compound 2. Sub G₀: represent cells with fragmented DNA, cell death; G₀/G₁: diploid cells (2N); S: DNA replication; G₂/M: cells with duplicate DNA content.