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Two-dimensional native gels showing dynamics of nucleosome sliding. Electrophoresis of 359-bp hsp70 mononucleosomes containing WT and mutant histones was first conducted in TE buffer (4% polyacrylamide gel). Entire gel lanes containing the separated nucleoprotein species identical to Fig. 1B were excised and applied horizontally for electrophoresis on 5% polyacrylamide gels in TGE buffer. The electrophoretic positions of N1–N4, N*, and free DNA are indicated.
