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. 2019 Apr 16;9:6165. doi: 10.1038/s41598-019-42127-8

Figure 2.

Figure 2

A CXCL12 promotes brown adipocyte activity. Mature CB-1 cells were treated with 100 nM of recombinant CXCL12 for 6 h. Bonferroni/Dunn test was used to compare the difference between the three groups. *P < 0.05, **P < 0.01 versus control. Values are shown as mean ± SD. (A) Changes in the expression of each gene when recombinant CXCL12 was added to brown adipocytes. (n = 3–4). Black, open, and stripe bar are shown as control, 10 nM, 100 nM of CXCL12, respectively. (B,C) Changes in expression of UCP-1 protein in CB-1 cells treated with the CXCL12 (n = 5). Black, open, and stripe bar are shown as control, 10 nM, 100 nM of CXCL12, respectively. (D,E) Oxygen consumption rate (OCR) when recombinant CXCL 12 was added to brown adipocytes (n = 20). Extracellular Acidification Rate (EACR) in CB-1 cells with CXCL12 (n = 20). Black arrow shows the point of addition of CXCL12. Black square, open circle, and black triangle are shown as control, CXCL12, and CL316,243. *P < 0.05. CL316,243 versus control, #P < 0.05 CXCL12 versus control. (F) Various mRNA expression levels in CB-1 cells treated with CXCL12 (n = 6). Black, open, and stripe bar are shown as control, CXCL12, and CL316,243.