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. 2019 Apr 16;9:6166. doi: 10.1038/s41598-019-42720-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation between trypan blue exclusion (TBE) viability and the ADP/ ATP ratio in the necrosis model The ADP/ATP ratio was strongly and negatively correlated with TBE viability (R² = 0.559, P < 0.001). However, our data suggested that the ADP/ATP ratio of hepatocytes of <0.12 (corresponding to TBE viability of > 60%) did not correlate with TBE viability (R² = 0.178, P = 0.172) (n = 12).