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. 2019 Apr 16;9:6166. doi: 10.1038/s41598-019-42720-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of the hepatocyte viability and function in the apoptosis model by conventional assays. (A) PE of the OA (dotted bar, 9.72 ± 3.43%, n = 8) and control groups (left hatched bar, 56.13 ± 5.11%, n = 8). *P < 0.001 in OA vs. control. (B) DNA quantity at 5 days after the plating of the OA (dotted bar, 5.65 ± 1.92 μg, n = 8) and control groups (left hatched bar, 9.76 ± 1.43 μg, n = 8). *P < 0.001 in OA vs. control. (C) The remaining ammonia concentration at 1, 2 and 3-h in the OA (solid line, 49.0 ± 7.22, 9.43 ± 4.34 and 3.66 ± 2.45%, respectively, n = 8) and control groups (dashed line, 37.91 ± 4.06, 7.47 ± 1.77 and 5.60 ± 2.76%, respectively, n = 8). *P < 0.001 at 1-h in OA vs. control.