Table 2.
PCR primers used in this study.
| Primers | aSequences | Restriction sites | PCR product size (bp) |
|---|---|---|---|
| nth-5F | CTTTAACTTTAGCCGCATC | 506 | |
| nth-5R | CGGGGTACCAATTTAAGCTCTGTTACAGGC | KpnI | |
| nth-3F | CGCGGATCCTAACTATCTTCATCAAGCCAT | BamHI | 562 |
| nth-3R | AATTTCTTTCTCTTTGTTCGAT | ||
| nth-Fc | CTAGTCTAGATTCTGTATCGCTAATGCTC | XbaI | 881 |
| nth-Rc | CTAGTCTAGACACTAGGCTTGTAAGGTTC | XbaI | |
| cj1083c-5F | GCCTTAGCAGATATCATCG | 502 | |
| cj1083c-5R | CGGGGTACCAGCTTGGTAAAAATTTGTGCTC | KpnI | |
| cj1083c-3F | CGCGGATCCAGAAAATGCGAACTTTACGAA | BamHI | 566 |
| cj1083c-3R | AATATTAGGCGTAAGAATGTC | ||
| uvrA-5F | GGACTTTTAATTGCTCCGAT | EcoRI | 541 |
| uvrA-5R | CGGGGTACCCTTCAGCATAAAGAGTTCCA | KpnI | |
| uvrA-3F | CGCGGATCCCGCTTTATATACTTGATGAACCT | BamHI | 665 |
| uvrA-3R | AAAGAATATTTCGCAAAACCA | ||
| uvrC-5F | GCCTTTATATCCGCAAGCA | 685 | |
| uvrC-5R | CGGGGTACCAGCAAAATAAGATCTAACACGA | KpnI | |
| uvrC-3F | CGCGGATCCAAGCTTGCAAATTTAGGAC | BamHI | 542 |
| uvrC-3R | CTTCATTTAAGCAACGCAT | ||
| aphA3-F | TATGGTACCCGCTTATCAATATATCTATAGAATG | KpnI | 1209 |
| aphA3-R | CGCGGATCCGATAATGCTAAGACAATCACTAAA | BamHI | |
| cat-F | CGGTGGTACCTGGAGCGGACAACGAGTAAA | KpnI | 817 |
| cat-R | CGCGGATCCTCAGTGCGACAAACTGGGATT | BamHI | |
| nth-HisF | CACCATCACCATCACGGATCCAAAAGAAATTTAGAAATCAAAGAAC | 624 | |
| nth-HisR | CCAAGCTCAGCTAATTAAGCTTTCATTTAAGTTCCTTATCTTTACTTTTG | ||
| cj1083c-HisF | GGATCGCATCACCATCACCATCACGGATCCACTGGAGCACAAATTTTTAC | 684 | |
| cj1083c-HisR | ACAGGAGTCCAAGCTCAGCTAATTAAGCTTTCATAAATCTTCCTTTAAAATTTTAATC |
aThe nucleotides in bold and underlined represent the restriction sites added to the primers (KpnI, BamHI, or XbaI) with three additional bases at the 5′ end to facilitate enzyme recognition and cleavage as suggested by the manufacturer (New England BioLabs, United States).