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. 2019 Apr 10;10:735. doi: 10.3389/fmicb.2019.00735

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Copyright © 2019 Panzer, Brych, Batschauer and Terpitz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electrophysiological characterization of UmOps1 and UmOps2. (A) Action spectra of UmOps1 and UmOps2. The mean relative pump activity and standard deviation of n measurements as indicated are given in dependence of the wavelength used for excitation. Both rhodopsins show highest activation at 532 nm. In these measurements, NaCl pH 5.0 was used as pipette solution to support proton pumping at low light intensities. Current–voltage relation of UmOps1 (B) and UmOps2 (C) in a range of +40 to –120 mV after excitation with a 532 nm laser in different extracellular solutions as indicated. Shown is relative pump activity (mean + standard deviation of n measurements as indicated) normalized to the value obtained in bath solution NaCl pH 7.4 at 0 mV clamp voltage. Note that pump activity of UmOps1 is highest at extracellular pH 5.0, which is remarkable for an outward directed proton pump, as the gradient is working against the pump.