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. 2019 Apr 10;10:735. doi: 10.3389/fmicb.2019.00735

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Copyright © 2019 Panzer, Brych, Batschauer and Terpitz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Life-cell fluorescence microscopic analysis of the localization of eGFP-tagged UmOps1 (A,B) and UmOps2 (C,D) after heterologous expression in U. maydis sporidia. Images were obtained with a 3D-SIM. Sporidia were either co-stained for mitochondria (SYTO^®59) and vacuoles (pHrodo^®Red; A,C), or for vacuolar membranes (FM4-64; B,D). Expression of UmOps1-eGFP was driven by the arabinose-inducible crg-promoter, expression of UmOps2-eGFP by the constitutive etef-promoter. Localization of UmOps2-eGFP after arabinose-induced expression is shown in Supplementary Figure S8. Note that UmOps1 is mainly located in the plasma membrane whereas UmOps2 is present in the vacuolar membranes. Scale bars represent 3 μm.