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. 2019 Apr 10;9:258. doi: 10.3389/fonc.2019.00258

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Copyright © 2019 Rather, Lin, Lin, Szekely and Bertino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry staining of JeKo-1 tumors extracted from mice in Figures 4, 5 against various biomarkers. The tumors were harvested from control, 1 and 5 mg/kg (i.p. weekly) M69-MMAE treatment groups from experiment four and Bortezomib and M69-MMAE combination (Bortezomib (0.75 mg/kg, i.p. weekly and M69-MMAE, 5mg/kg i.p. twice weekly) from experiment five. Ki67 staining showing proliferation of JeKo-1; and Cleaved caspase-3 showing the apoptotic cell death. The tonsil tissue was used as a positive control for various IHC staining.