Figure 1.

SFG Rickettsia trigger reprogramming in THP-1 macrophages early in infection. (A,B) Volcano plots of log₂ fold change ratio of the expression levels in R. conorii- (A) and R. montanensis- (B) infected THP-1 macrophages over that in uninfected cells plotted against the -log₁₀ (q-value). Statistically differentially expressed genes found in increased abundance and in decreased abundance are represented in red and green, respectively (FDR < 0.05). See also Tables S1, S2. (C,D) Validation of RNA-seq data by comparing the transcriptional fold changes of 11 randomly selected genes determined by RNA-seq and an independent method (q-RT-PCR) for R. conorii- (C) and R. montanensis- (D) infected cells. Pearson analysis of correlation and respective significant test (two-tailed) were performed in GraphPad Prism. R. conorii-infected: r = 0.9208, N = 11, p < 0.0001 and R. montanensis-infected: r = 0.6437, N = 11, p < 0.05. Gene labeling: 1-B2M; 2-BTG2; 3-CD69; 4-EGR1; 5-EMC7; 6-G6PD; 7-IER3; 8-KLF10; 9-MTRNR2L6; 10-OTUD1; 11-PP1R15A. See also Table S3.