Table 2.

Primers and probes.

Group	Target species	Target gene	Primer/ probe name	Sequence (5′-3′)	Length (nt)	Tm (C)	PCR product size (bp)
A	O12	wzm	wl71881	AAAGGAAGGCTATGCGTTC	21	55.2	184
			wl71882	GAAAGGTTCATTAGTCTGGGA	19	54.7
			OA-5521	ACTCCACCATCCAAGAGAAAT	21	53.7
	O25	wzm	wl71869	AGAGACTTTCCTCAAATGGTAA	22	53.1	172
			wl71870	TACACCTCTGGAACTTTACCT	21	51.0
			OA-5515	AGAGCCCCGCTGATAGG	17	55.4
	O26	wzx	wl71879	AAATGTCACCATCTTTACGG	20	52.6	351
			wl71880	GCACTCTGACCTGCCTGATA	20	55.7
			OA-5520	GTATACCAGCGCGAGTACATTT	22	57.0
	O33	wzy	wl71871	TAAGCAGAAACCGTCTAACC	20	52.5	256
			wl71872	CAGCTCAGTAGGTACGAGAAAT	22	53.7
			OA-5516	CAGAAACCGTCTAACCACTACAT	23	55.9
	O75	wzy	wl71875	TAACTCTTTTCTATGTGCCGA	21	53.2	82
			wl71876	GGCATTACACAGAGCAAGAT	20	52.5
			OA-5518	ATGATCGGCCATCGTCAT	18	56.0
	O76	wzy	wl71877	CACATATGGAAAAAACTGGG	20	53.1	205
			wl71878	AAGAAACAGCCGACCAGATA	20	54.9
			OA-5519	GGCGCACGTATCGAAGG	17	57.8
B	O2	wzx	wl72935	AATGGATTTGTGCTTTTTCTT	21	54.0	263
			wl72936	GCAAGTTGCCACTTAGTGTTA	21	53.5
			OA-5851	CAGGCCCATTGAACCAGT	18	55.8
	O10	wzx	wl72937	AATAATAGTGGCTGGTTAATGTT	23	53.2	231
			wl72938	AAATGCCGTTCCGAGTAT	18	52.7
			OA-5852	AACCAAAGCGGGTGAGATA	19	55.4
	O23	wzy	wl72931	CTACTGAATCCAACCACGC	19	53.4	192
			wl72932	ATTTTGTGGATTATACATTGGA	22	52.2
			OA-5849	CACCAAGCCAAGTCGAGAA	19	56.6
	O32	wzy	wl72925	AGTCAATAACATACCCAAACG	21	52.3	290
			wl72926	AGAGAGCCCTTCTATTCCAA	20	53.4
			OA-5846	GTCGCAGACTGTTGACTAAGAA	22	54.9
	O34	wzy	wl72927	GGTCTCCGCAAAAGATAGTC	20	54.0	317
			wl72928	TTTATCGCTTACTCTTGCTCA	21	53.6
			OA-5847	TTAACGCCAACCAGGTAGTAG	21	55.3
	O66	wzm	wl72933	CACATTCGTGTTTAGTGTGGT	21	53.2	214
			wl72934	TCCAAAACTTGAGAACACCA	20	53.5
			OA-5850	AGGCAGCGGTTCAAAGC	17	56.6