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. 2019 Apr 10;10:357. doi: 10.3389/fneur.2019.00357

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Copyright © 2019 Hwang, Choi and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro assays for neurogenesis following EPO, hUCBC and hUCBC+EPO treatment using OGD-injured neural stem cells. (A) Cell viability of EPO concentration in OGD-injured neural stem cells (B) cell viability was decreased by OGD, and increased by EPO and hUCBC and the most by hUCBC+EPO. (C) Reverse transcription polymerase chain reaction (RT-PCR) for expression of Sox2 and Nestin. Sox2 gene expression was significantly increased by EPO or hUCBC treatment, and it was most increased by hUCBC+EPO. (D) Western blotting for expression of Sox2 and Nestin. Sox2 was upregulated by hUCBC and hUCBC+EPO treatment. Data represent mean ± SEM. N = 3 per group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.005 vs. OGD-injured cells. ^#P < 0.05 for inter-treatment effect comparison.