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. 2019 Apr 10;10:744. doi: 10.3389/fimmu.2019.00744

Figure 6.

Figure 6

Kctd9 depletion downregulated NK cell-related transcription factor expression. (A) Real-time PCR analysis for Ets1, Nfil3 (encodes E4bp4), Eomes, Tbx21 (encodes T-bet), Id2, and Tox expression by purified NK cells from spleen of WT and Kctd9−/− mice. (B) Expression of Eomes by rNKPs in BM from WT and Kctd9−/− mice. (a) Flow cytometric analysis for Eomes in rNKPs. Plots represent rNKPs. (b) Intensity of Eomes expression in total rNKPs. (C,D) Expression of Eomes by CD3−NKp46+ NK cells (C) and subsets defined by CD11b/CD27 expression in CD3−NKp46+ NK cells (D) from WT and Kctd9−/− BM. (a) Flow cytometric analysis for expression of Eomes. Plots represent CD3−NKp46+ NK cells (C) or subsets of CD3−NKp46+ NK cells (D). Frequency of gated population is indicated in plots. (b,c) The percentages and the intensity of Eomes expression of CD3−NKp46+ NK cells (C) and subsets of CD3−NKp46+ NK cells (D) positive for Eomes. All results were representative of three independent experiments. Four to six mice of each genotype were used in each experiment. Error bars indicate standard deviation. *P < 0.05, **p < 0.01.