Table 4.

Summary of key characteristics of JO4-derivatives.

Characteristic	JO-4	GB3V239D	GB7V239D/JOC-x
pI	8.91	9.11	7.30
Production level (mg/L)	1–5	1–3	10–15
Ave. endotoxin (EU/mg)	691,300 K	80,500	2,500
% aggregated by SECA	90/94%	55/43%	12/4%
Affinity to DSG2 (nM)	0.58	0.11	1.2
DSG2 shedding (fold over PBS treated at 48 h)	1.44	1.52	1.39
VIA IC50 (μg/mL)	0.067	0.029	0.032

^AFirst value reported is untreated, second value is following reduction with DTT.