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. 2019 Apr 16;9:6146. doi: 10.1038/s41598-019-42484-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relevant features of the P12294 promoter region. (A) Genetic organization of the chromosome region that contains OG1RF_12294. Coordinates of the translation start and stop codons are indicated. Stem-loop elements represent potential transcriptional terminators. Arrows upstream of the genes represent promoters. The nucleotide sequence of the region spanning coordinates 2425780 to 2425601 is shown. The stop codon (TAA) of OG1RF_12295 and the start codon (ATG) of OG1RF_12294 are indicated in boldface letters. IR: inverted-repeat. SD: Shine-Dalgarno sequence. The main sequence elements (−35 box and −10 box) of the P12294 promoter are indicated. The MafR binding site defined in this work is shown (shadowed box). Genes OG1RF_12294 and OG1RF_12295 correspond to genes EF3014 and EF3015 in E. faecalis strain V583. (B) Bendability/curvature propensity plot of the region spanning coordinates 2425817 to 2425548. The location of the P12294 core promoter, the start codon of OG1RF_12294 and the MafR binding site are indicated.