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. 2019 Apr 16;9:6146. doi: 10.1038/s41598-019-42484-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of plasmid-encoded MafR on the activity of the P11486 promoter. Five regions from the OG1RF chromosome were inserted independently into the SacI site of pASTT. The coordinates of such regions are indicated. Gene tetL: tetracycline resistance determinant. Gene gfp: green fluorescent protein. The T1T2 box represents the tandem transcriptional terminators T1 and T2 of the Escherichia coli rrnB rRNA operon. The arrow represents the −35 element of the P11486 promoter. The intensity of fluorescence (arbitrary units) corresponds to 0.8 ml of culture (OD₆₅₀ = 0.4). In each case, three independent cultures were analysed. N.D.: non-determined.