Fig. 5. Isolation and characterization of tumor cells from the vitreous cavity of mice.

a Representative images of glioblastoma cells. b Representative images of glioblastoma cells that were stained using DAPI and antibodies specific for GFAP and human mitochondria. GBL-28N and GBL-37N, cells isolated from mice at 4 weeks after the intravitreal injection of GBL-28 and GBL-37 cells that underwent normal culture conditions. GBL-28H and GBL-37H, cells isolated from mice at 4 weeks after the intravitreal injection of GBL-28 and GBL-37 cells that underwent hypoxia treatment for 4 h before injection. Scale bar: 100 μm