Figure 3.

The effect of iron on the growth of the aft mutant strains. Overnight cultures of the wild-type strain and each of the aft-mutant strains were grown in liquid CM(−Ura) with glucose medium. Each strain was grown in duplicate, with one of each pair being supplemented with FeCl₂ (100 μM). Iron supplementation in the pregrowth medium is indicated by “+ Fe.” The cells were washed, and 10-fold serial dilutions were spotted onto (A) complex medium (YPD) agar plates with or without supplemented FeCl₂ (100 μM) or (B) synthetic medium (CMD) agar plates with or without supplemental FeCl₂ (100 μM) or BPS (10 μM). Plates were incubated at 30°C and, in the case of one YPD plate, under anaerobic conditions.