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. 2001 Dec 4;98(25):14322–14327. doi: 10.1073/pnas.261381198

Figure 4.

Figure 4

S1 nuclease protection assays to quantify mRNA levels of genes activated by the AFT2-1up allele. RNA was isolated from (A) the aft2Δ strain and (B) the aft1Δ strain transformed with pRS416 (−), pAFT2 (+), and pAFT2-1up (up) and grown in CM(−Ura) with glucose medium to mid-log phase. The upper band for each sample is the specified gene, and the lower band the calmodulin loading control (CMD1).