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Iron uptake in the aft mutants. The level of 59Fe uptake was analyzed in the following cultures. (A) The wild-type strain and the aft-deletion strains grown to mid-log phase in synthetic medium (CMD) with BPS (100 μM). (B) The wild-type strain and the aft1Δ strain containing either pRS416 (WT, 1Δ) or pAFT2-1up (WT/up, 1Δ/up) grown to mid-log phase in CMD(−Ura) medium.
