Table 1.
Genes activated by the AFT2-1up allele
| Target gene | Cy5/Cy3 ratio | Function |
|---|---|---|
| FIT3 | 8.8 | Cell wall protein |
| YOL154w | 8.8 | Zn-metalloprotease-like protein |
| ZRT1 | 6.2 | High affinity zinc transporter |
| FIT1 | 5.7 | Cell wall protein |
| ECM4 | 5.0 | Cell wall structure/biosynthesis |
| YOL083w | 4.2 | Unknown |
| UBC8 | 4.1 | Ubiquitin-conjugating enzyme |
| YJR078w | 3.6 | Similar to indoleamine 2,3-dioxygenase |
| MRS4 | 3.5 | Member of mitochondrial carrier family |
| TIS11 | 3.3 | Similar to mammalian TIS11 family |
| FTR1 | 3.3 | High affinity iron transporter |
| FTH1 | 3.2 | Vacuolar iron transporter |
| LAP4 | 3.0 | Vacuolar aminopeptidase I |
| PRB1 | 2.9 | Vacuolar protease B |
| GRX4 | 2.8 | Glutaredoxin |
| AHP1 | 2.7 | Alkyl hydroperoxide reductase |
| HCR1 | 2.6 | Putative component of eIF3 |
| FET5 | 2.6 | Vacuolar multicopper oxidase |
| PEP4 | 2.6 | Vacuolar proteinase A |
| YDL124w | 2.5 | Unknown |
| FRE1 | 2.2 | Cell surface iron reductase |
| SMF3 | 2.0 | Vacuolar iron transporter |
Transcripts of these genes were at least 2-fold more abundant in the strain containing the AFT2-1up allele compared with the control strain. The value shown is the Cy5/Cy3 ratio (which reflects the ratio of the transcripts' abundance in the two strains) measured in one experiment at the Stanford Microarray Facility. The same genes were identified in two similar experiments conducted at the University of Utah Microarray Facility.