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. 2019 Apr 16;9:6133. doi: 10.1038/s41598-019-42647-3

Figure 1.

Figure 1

The chlamydial candidate T3S effector CT105 is delivered by C. trachomatis into the cytoplasm of infected cells. HeLa cells were either left uninfected (UI) or infected by C. trachomatis L2/434-derived strains harboring the indicated plasmids, encoding candidate T3S effectors (CT053, CT429, CT105, CT082, CT849) with a 2HA epitope tag at their C-termini. (A) At 40 h p.i., whole cell extracts were analyzed by immunoblotting with antibodies against HA, MOMP (bacterial loading control) and α-tubulin (HeLa loading control) using SuperSignal West Pico detection kit (Thermo Fisher Scientific), or SuperSignal West Femto detection kit (Thermo Fisher Scientific) for CT849-2HA. Asterisks indicate the bands likely corresponding to the full-length proteins. Whole immunoblots are presented in Supplementary Fig. S1. (B) At 20 h p.i., cells were fixed with 4% (w/v) PFA, stained with DAPI (blue), immunolabeled with antibodies against HA (red) and C. trachomatis MOMP (green), and appropriate fluorophore-conjugated secondary antibodies, and imaged by fluorescence microscopy. Scale bars, 10 µm.