Figure 2.

CT105 colocalizes with the Golgi complex in C. trachomatis-infected cells. HeLa cells were infected by C. trachomatis L2/434 encoding CT105-2HA. At the indicated times p.i., cells were fixed with 4% (w/v) PFA, immunolabeled with antibodies against HA (red), C. trachomatis MOMP (gray), GM130 (green), and TGN46 (blue), and appropriate fluorophore-conjugated secondary antibodies. In the area delimited by a white square (left-side panels) images were zoomed (middle and right-side panels). Immunolabeled cells were examined by confocal fluorescence microscopy, and images correspond to single z sections. Scale bars, 10 µm.