Fig. 5.

Truncated PRPH variants generate protein inclusions. SW13 cells were transfected with a wild-type (PRPH WT) using n = 332 cells, b 4bp-splice variant (PRPH 4 bp) using n = 545 cells, or c intron retention splice-variant (PRPH Intron), cultured for 48 h and labeled with an antibody raised against peripherin and counterstained with DAPI (4′,6-diamidino-2-phenylindole) for nuclear visualization, using n = 177 cells. Scale bar represents 10 µm. d Peripherin filaments and inclusions were quantified from the three independent experiments (n = 177–545). Inclusions were subdivided by size; fibrous inclusions ≥5 µm, large inclusions 1.5–5 µm, and small inclusions ≤1.5 µm (n = 177–545). Error bars represent standard deviations (SD)