FIGURE 1.

bβ-arrestin-2 translocation to V₂R, D₂R, CB₁, and CB₂. bβ-Arrestin-2 translocation to plasma membrane in cells expressing (A) V₂R stimulated with AVP, (B) D₂R with or without co-expressed GRK2 stimulated with dopamine, (C) CB₁ stimulated with CP55,940, 2-AG, AEA, BAY, THC, and WIN55,212-2, (D) CB₁ with co-expressed GRK2 stimulated with CP55,940 and 2-AG, (E) CB₂ stimulated with CP55,940, 2-AG, JWH-015, JWH-133, THC, and WIN55,212-2, and (F) CB₂ with co-expressed GRK2 stimulated with CP55,940 and 2-AG. (G) Receptor expression of V₂R, D₂R, D₂R+GRK2, CB₁, CB₁+GRK2, CB₂, CB₂+GRK2, and no-receptor transfected controls in cells used in experiments (A–G). Receptor expression per cell was quantified in cells positive for expression by ICC. (H) bβ-Arrestin-2 translocation to plasma membrane in mock-transfected cells. Error bars are ±standard deviation of representative data with three technical replicates (A–F), or standard error of the means from three independent biological replicates (G,H).