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. 2019 Apr 10;10:350. doi: 10.3389/fphar.2019.00350

FIGURE 2.

FIGURE 2

hβ-arrestin-2 translocation to V2R, D2R, CB1, and CB2. hβ-Arrestin-2 translocation to plasma membrane in cells expressing (A) V2R stimulated with AVP, (B) D2R with or without co-expressed GRK2 stimulated with dopamine, (C) CB1 stimulated with CP55,940, 2-AG, AEA, BAY, THC, and WIN55,212-2, (D) CB1 with co-expressed GRK2 stimulated with CP55,940 and 2-AG, (E) CB2 stimulated with CP55,940, 2-AG, JWH-015, JWH-133, THC, and WIN55,212-2, and (F) CB2 with co-expressed GRK2 stimulated with CP55,940 and 2-AG. (G) Receptor expression of V2R, D2R, D2R+GRK2, CB1, CB1+GRK2, CB2, and CB2+GRK2 in cells used in experiments (A–G). Receptor expression was quantified in cells positive for expression by ICC. (H) hβ-Arrestin-2 translocation to plasma membrane in mock-transfected cells. Error bars are ±standard deviation of representative data with three technical replicates (A–F), or standard error of the means from three independent biological replicates (G,H).