DNMT protein levels and global DNA methylation levels in the early postnatal HR versus LR amygdala and hippocampus. A, LR rats exhibited higher levels of DNA methylation (5-methylcytosine; n = 5–7) and DNMT1 (n = 5–7), DNMT3a (n = 5–7), and DNMT3b (n = 7) protein levels in the amygdala at postnatal day (P)7 compared with HRs, with little to no difference at the later ages examined (P14 and P21). B, In the hippocampus, there were no HR/LR differences in DNA methylation markers (global DNA methylation, n = 5; DNMT1, n = 5–7; DNMT3a, n = 4–7; DNMT3b, n = 7) at any age examined. There were no HR/LR hydroxymethylation differences in the amygdala or hippocampus at any age examined. *p < 0.05 for HR versus LR comparisons. All sample sizes are per age and phenotype.