Figure 8.

Increasing synaptic drive and limiting EGTA in the loading pipette unveils CP-AMPAR-mediated Ca²⁺ transients in mature principal cells. A1, OGB1 was used to record Ca²⁺ transients from the principal cells of the MNTB induced by midline stimulation at a frequency of 100 Hz from P27–P30 mice. EGTA was excluded from the loading pipette and the stimulation time was increased to 1 s. Midline stimulation (gray box denotes stimulation) produced a Ca²⁺ response (control, black) and application of IEM-1460 inhibited this response (IEM, red). The Ca²⁺ response to a 500 ms postsynaptic voltage-clamp step depolarization is shown for comparison (green). A2, Region outlined by dotted lines in A1 shown at higher magnification. A3, Summary data of the peak Ca²⁺ responses recorded in the P27–P30 group in control conditions or after the addition of IEM-1460 (paired t test; n = 8, p = 0.0170). ns, nonsignificant, *p < 0.05.