Fig. 3.
The effects of IL-1Ra on cell apoptosis after intestinal I/R. (A) Intestinal sections were stained with TUNEL (green) and DAPI (blue). Representative photographs of TUNEL-positive cells. (B) Quantitative analyses of TUNEL-positive cells. (C) The protein expression levels of Bcl-xl, Bax, Cleaved-caspase-3 and Caspase-3 were analyzed by Western blot. (D, E) The gray value of band was scanned by optical densitometry, the ratio value was statistically analyzed. Data were shown as the mean ± SD (n = 3). #p < 0.05 versus S; *p < 0.05 versus I/R.