Skip to main content
. 2019 Mar 29;13:58–66. doi: 10.1016/j.omto.2019.03.008

Figure 1.

Figure 1

PCSCs Have Self-Renewal Potential In Vitro

(A) Sphere formation assay in 3-week DU145 sphere-cultured cells (n = 60 wells). (B) Sphere formation assay in 3-month DU145 sphere-cultured cells. For 1 cell/well assay, experiment was repeated 4 times. (C) Extreme limiting dilution analysis (ELDA) comparing 3-week DU145 sphere cultures (group A, black) and 3-month DU145 sphere cultures (group B, red) (p < 0.001). (D) Morphology of 3-week DU145 sphere plated at 1,000 cells/well. (E) Morphology of 3-month DU145 sphere plated at 1 cell/well. (F) Comparison of sphere diameters between 3-week DU145 spheres (1,000 cells/well) and 3-month DU145 spheres (1 cell/well). n = 3, mean ± SD, ***p < 0.0001. (G) Sphere formation assay of TRAMP-C2 parental cells (n = 60 wells). (H) Sphere formation assay of TRAMP-C2 sphere-cultured cells. For 1 cell/well assay, experiment was repeated 3 times. (I) ELDA comparing TRAMP-C2 parental (group A, black) and TRAMP-C2 sphere-cultured cells (group B, red) (p < 0.001). (J) Morphology of sphere in 100 cells/well of TRAMP-C2 parental. (K) Morphology of sphere in 1 cell/well of TRAMP-C2 sphere culture. (L) Comparison of sphere diameters between TRAMP-C2 parental (100 cells/well) and TRAMP-C2 sphere cultures (1 cell/well). There is no statistical difference. Scale bars, 100 μm.